Fractionation of human urine by gel chromatography.

High-resolution anion-exchange chromatography of human urine can resolve more than 150 constituents that absorb ultraviolet light. Prefractionation of urine on Sephadex G.10 helped us identify the constituents and simplified the anion-exchange chromatogram. The material associated with the 13 peaks obtained by gel chromatography was pooled and concentrated into six fractions, which were subsequently analyzed by highresolution anion-exchange chromatography. Each of these fractions contained from 13 to 63 ultraviolet-absorbing constituents.